How is amniocentesis performed?

In amniocentesis, amniotic fluid is aspirated from the uterine cavity ultrasound guidance. It is usually done after 15 weeks of pregnancy by transabdominal route.

Abdomen is cleaned and draped under aseptic conditions. Site of needle insertion is identified (Non placental site).22G spinal needle is introduced into the uterus under ultrasound guidance and amniotic fluid is aspirated. First 2ml of fluid is discarded to avoid maternal cell contamination.

A minimum of 20 ml of fluid is required to achieve a valid result Injection Anti-D is given to Rh negative mother within 24 hrs of performing procedure to prevent alloimmunization.

Consult Dr. P. Madhavi Latha at pregnancy scanning centers in Vijayawada

What are the Complications of amniocentesis procedure?

  1. Procedure related fetal loss rate is 0.1 to 1%
  2. Membrane rupture 1-2%
  3. Chorioamnionitis <0.1%
  4. Fetal injury and serious maternal complications are rare are uncommon.

Laboratory aspects of CVS and amniocentesis:

  1. Procedure related fetal loss rate is 0.1 to 1%
  2. Membrane rupture 1-2%
  3. Chorioamnionitis <0.1%
  4. Fetal injury and serious maternal complications are rare are uncommon.

How is fetal blood sampling performed?

In fetal blood sampling, blood is aspirated from the umbilical vein ultrasound guidance. It is usually done after 18 weeks of pregnancy by transabdominal route.

Abdomen is cleaned and draped under aseptic conditions. Local anesthesia is given at the site of needle insertion is identified (cord insertion site).20G 15cm needle is introduced into the umbilical vein under ultrasound guidance and fetal blood is aspirated.

Procedure related fetal loss rate is 1-2%

Factors associated with increased fetal loss

  1. Fetal anomalies
  2. GA <24weeks
  3. IUGR

Genetic tests performed on fetal cells :

  1. Fetal Karyotype
  2. Fluorescent in situ hybridization (FISH)
  3. QF-PCR
  4. Microarray
  5. Prenatal Bobs
  6. Mutational analysis .

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